Fast deep confocal imaging
Beyond mechanical constraints
PhaseStreak is designed to adapt to different samples and imaging requirements. It combines fast acquisition, flexible control over illumination and strong optical sectioning in a robust platform designed for minimal routine maintenance.
Rethinking confocal imaging for speed and flexibility
PhaseStreak is designed to adapt to different samples and imaging requirements. It combines fast acquisition, flexible control over illumination and strong optical sectioning in a robust platform designed for minimal routine maintenance.
SOLID-STATE ARCHITECTURE
No mechanical scanners or physical pinholes.
230 FPS
Camera-limited confocal and widefield acquisition.
3 MODES. INSTANT SWITCHING
Confocal, widefield and brightfield imaging.
23.1 mm FOV
Imaging across a large field of view.
ADJUSTABLE SECTIONING
Electronically tune the balance between background rejection and signal collection.
300+ µm DEEP
Optical sectioning across thick samples.
AOD-based scanning camera-based confocal sectioning
PhaseStreak scans a focused excitation line across the sample using an acousto-optic deflector synchronised with the rolling shutter of a high QE sCMOS camera. The active sensor rows form an electronically adjustable confocal detection slit that suppresses out-of-focus fluorescence and enables optimizing the balance between background rejection and signal collection for each sample.
AOD-BASED SCANNER
Fast, precise and inertia-free control of excitation laser beam.
PARALLEL LINE ACQUISITION
An entire line of pixels is acquired simultaneously, enabling confocal imaging at demonstrated speeds of up to 230 fps.
LOW-MAINTENANCE ARCHITECTURE
With no mechanical scanners or physical pinholes, PhaseStreak minimizes alignment drift, mechanical wear and avoids dust-related pinhole contamination.
See deeper
Preserve contrast
As imaging depth increases, scattering and out-of-focus fluorescence progressively reduce contrast. PhaseStreak combines selective confocal detection with efficient signal collection to preserve image clarity throughout thick samples.
3D volumetric view of a cleared mouse liver tissue down to 400 µm. Sample: 550 µm cleared mouse liver section; SYTOX Orange/nuclei (green), Alexa Fluor 647-TUJ1/nervous system (magenta).
Taking care of your cells
Following biological dynamics over time requires a careful balance between image quality and sample exposure. PhaseStreak gives you direct control over where excitation is delivered and how fluorescence is collected, helping you adapt that balance to each experiment.
>300 SIGNAL-TO-BACKGROUND RATIO
Achieve high image contrast with lower laser power.
DISTRIBUTED LINE EXCITATION
Distributed line illumination and efficient signal acquisition minimise the illumination dose required for high-contrast imaging.
ROI ILLUMINATION
Illuminate only the area you need while increasing acquisition speeds up to the kHz range.
TUNABLE SIGNAL COLLECTION
Adapt optical sectioning and signal collection to each sample.
Multicolor confocal image of BPAE cells acquired with PhaseStreak. Nuclei are labelled with DAPI (magenta), F-actin with Alexa Fluor 488 phalloidin (cyan) and mitochondria with MitoTracker Red CMXRos (yellow). Sample: Thermo Fisher FluoCells Prepared Slide #1, F36924. Objective: Plan Apo 60x WI NA1.2
Control Automate Acquire
All from one interface
SharpEye brings all PhaseStreak controls and automated acquisition workflows into a single, self-contained interface, combining streamlined operation with the flexibility of the Micro-Manager ecosystem.
INSTANT MODE
SWITCHING
Switch between confocal, widefield and brightfield imaging in one click.
ELECTRONIC SECTIONING CONTROL
Adjust the confocal detection aperture and tune signal collection directly from the interface.
FOUR-CHANNEL
ACQUISITION
Control laser power independently at 405, 473, 561 and 639 nm and configure sequential multichannel acquisition.
AUTOMATED
ACQUISITION
Configure and run multichannel Z-stacks, time-lapse recordings and acquisition sequences from the same workspace.
Compatible with established Fiji/ImageJ visualisation and analysis workflows.
Add confocal imaging to your setup
PhaseStreak integrates via the camera port of compatible inverted microscopes and can also be combined with SENSOCELL+ for correlated force and fluorescence measurements.
- CONFOCAL ADD-ON FOR INVERTED MICROSCOPES
- INTEGRATION WITH SENSOCELL+
Phasestreak at a glance
* Acquisition speed is camera-limited. Demonstrated confocal imaging at 230 fps using a Tucsen Aries 6506 camera in Speed mode over 2400 × 2048 pixels, corresponding to 20.5 mm diagonal field of view.
** Demonstrated with a Hamamatsu ORCA-Fire camera over its full 4432 × 2368 pixel sensor area.
Experience a new approach to confocal imaging
Discover how PhaseStreak’s solid-state architecture brings greater speed, flexibility and control to your imaging workflows.
